Characterization of a putatively vesicular binding site for [3H]MPP+ in mouse striatal membranes
Identifieur interne : 002E80 ( Main/Exploration ); précédent : 002E79; suivant : 002E81Characterization of a putatively vesicular binding site for [3H]MPP+ in mouse striatal membranes
Auteurs : M. Del Zompo [Italie] ; M. P. Piccardi [Italie] ; S. Ruiu [Italie] ; G. U. Corsini [Italie] ; A. Vaccari [Italie]Source :
- Brain Research [ 0006-8993 ] ; 1992.
English descriptors
- KwdEn :
- Teeft :
- Adenosine triphosphate, Appropriate assay conditions, Assay, Binding site, Binding sites, Brain area, Brain research, Chromaffin granules, Chronic parkinsonism, Dopamine, Dopaminergic neurons, High affinity, Ligand program, Monoamine, Monoamine oxidase, Mouse brain, Mouse brains, Mouse striatal membranes, Mouse striatum, Mptp, Pharmacol, Potent displacers, Present assay conditions, Right striatum, Specific binding, Striatal, Striatum, Subcellular distribution, Synaptic vesicles, Synaptosomal fraction, Toxic metabolite, Vesicle, Vesicular monoamine transporter.
Abstract
Abstract: [3H] N-methyl-4-phenylpyridinium ion (MPP+) binds with fully reversible, high affinity process to a population of sites mainly localized in the mouse striatum (Bmax = 168 ± 15fmol/mg protein, KD = 1.4 ± 0.4nM). The majority of specifically-bound radioactivity was localized in the synaptosomal fraction. Unilateral, striatal denervation with 6-hydroxydopamine (6-OHDA) markedly (by 65–70%) decreased the number of [3H]MPP+ sites. Besides dopamine, the vesicular markers tyramine, tetrabenazine and reserpine inhibited [3H]MPP+, while mazindol was a poor displacer. Adenosine triphosphate (ATP) and Mg2+-ions did not affect [3H]MPP+ binding. It is concluded that these sites may represent a marker of striatal storage vesicles for dopamine.
Url:
DOI: 10.1016/0006-8993(92)90677-2
Affiliations:
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The majority of specifically-bound radioactivity was localized in the synaptosomal fraction. Unilateral, striatal denervation with 6-hydroxydopamine (6-OHDA) markedly (by 65–70%) decreased the number of [3H]MPP+ sites. Besides dopamine, the vesicular markers tyramine, tetrabenazine and reserpine inhibited [3H]MPP+, while mazindol was a poor displacer. Adenosine triphosphate (ATP) and Mg2+-ions did not affect [3H]MPP+ binding. It is concluded that these sites may represent a marker of striatal storage vesicles for dopamine.</div></front></TEI><affiliations><list><country><li>Italie</li></country><region><li>Toscane</li></region><settlement><li>Pise</li></settlement></list><tree><country name="Italie"><noRegion><name sortKey="Del Zompo, M" sort="Del Zompo, M" uniqKey="Del Zompo M" first="M." last="Del Zompo">M. Del Zompo</name></noRegion><name sortKey="Corsini, G U" sort="Corsini, G U" uniqKey="Corsini G" first="G. U." last="Corsini">G. U. Corsini</name><name sortKey="Piccardi, M P" sort="Piccardi, M P" uniqKey="Piccardi M" first="M. P." last="Piccardi">M. P. Piccardi</name><name sortKey="Ruiu, S" sort="Ruiu, S" uniqKey="Ruiu S" first="S." last="Ruiu">S. Ruiu</name><name sortKey="Vaccari, A" sort="Vaccari, A" uniqKey="Vaccari A" first="A." last="Vaccari">A. Vaccari</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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